Difference between revisions of "Part:BBa K3187022"

 
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<html>
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                <h3>Profile</h3>
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                <table style=“width:80%“>
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                <tr>
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                <td><b>Name</b></td>
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                <td> sfGFP (superfoler green fluorescent protein) </td>
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                </tr>
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                <tr>
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                <td><b>Base pairs</b></td>
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                <td> 714</td>
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                </tr>
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 +
                <tr>
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                <td><b>Molecular weight</b></td>
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                <td>26.8 kDa</td>
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                </tr>
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                <tr>
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                <td><b>Origin</b></td>
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                <td> <i> Aequorea victoria</i> </td>
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                </tr>
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 +
         
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                <tr>
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                <td><b>Properties</b></td>
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                <td> very fast maturing variant of GFP</td>
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                </tr>
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                </table>
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                <h3> Usage and Biology</h3>
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<p>
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GFP is a fluorescent protein originally isolated from the jellyfish <i>Aequorea victoria</i>.
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sfGFP is a variant of this protein. It has a short maturing time of 13.6&nbsp;min instead of 36.1&nbsp;min of the original protein variant, has an extinction maximum at 485&nbsp;nm and an emission maximum at 510&nbsp;nm
 +
 
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<sup id="cite_ref-1" class="reference">
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                            <a href="#cite_note-1">[1]
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                            </a>
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                    </sup>
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<sup id="cite_ref-2" class="reference">
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                            <a href="#cite_note-2">[2]
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                            </a>
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                    </sup>
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<sup id="cite_ref-3" class="reference">
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                            <a href="#cite_note-3">[3]
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                            </a>
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                    </sup>.
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</p>
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                    <h2>References</h2>
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                    <ol class="references">
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                        <li id="cite_note-1">
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                            <span class="mw-cite-backlink">
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                                <a href="#cite_ref-1">↑</a>
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                            </span>
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                            <span class="reference-text">
 +
                            Jean-Denis Pédelacq, Stéphanie Cabantous, Timothy Tran, Thomas C Terwilliger, Geoffrey S Waldo, Engineering and characterization of a superfolder green fluorescent protein, Nature Biotechnology volume24,pages79–88 (2006)
 +
                            <a rel="nofollow" class="external autonumber" href="">[1] </a>
 +
                            </span>
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                        </li>
 +
                   
 +
                        <li id="cite_note-2">
 +
                            <span class="mw-cite-backlink">
 +
                                <a href="#cite_ref-2">↑</a>
 +
                            </span>
 +
                            <span class="reference-text">
 +
                                FPbase: Superfolder GFP, last visited: 6.10.2019
 +
                            <a rel="nofollow" class="external autonumber" href="https://www.fpbase.org/protein/superfolder-gfp/">[2] </a>
 +
                            </span>
 +
                        </li>
 +
 
 +
 
 +
<li id="cite_note-3">
 +
                            <span class="mw-cite-backlink">
 +
                                <a href="#cite_ref-3">↑</a>
 +
                            </span>
 +
                            <span class="reference-text">
 +
                                Balleza, E., Kim, J. M., & Cluzel, P. (2018). Systematic characterization of maturation time of fluorescent proteins in living cells. Nature methods, 15(1), 47–51.
 +
                            <a rel="nofollow" class="external autonumber" href="https://dx.doi.org/10.1038%2Fnmeth.4509">[3] </a>
 +
                            </span>
 +
                        </li>
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 +
 
 +
                    </ol>
 +
                   
 +
           
 +
 
 +
</html>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 12:53, 16 October 2019


Superfolder Green Fluorescence Protein

Profile

Name sfGFP (superfoler green fluorescent protein)
Base pairs 714
Molecular weight 26.8 kDa
Origin Aequorea victoria
Properties very fast maturing variant of GFP

Usage and Biology

GFP is a fluorescent protein originally isolated from the jellyfish Aequorea victoria. sfGFP is a variant of this protein. It has a short maturing time of 13.6 min instead of 36.1 min of the original protein variant, has an extinction maximum at 485 nm and an emission maximum at 510 nm [1] [2] [3] .

References

  1. ↑ Jean-Denis Pédelacq, Stéphanie Cabantous, Timothy Tran, Thomas C Terwilliger, Geoffrey S Waldo, Engineering and characterization of a superfolder green fluorescent protein, Nature Biotechnology volume24,pages79–88 (2006) [1]
  2. ↑ FPbase: Superfolder GFP, last visited: 6.10.2019 [2]
  3. ↑ Balleza, E., Kim, J. M., & Cluzel, P. (2018). Systematic characterization of maturation time of fluorescent proteins in living cells. Nature methods, 15(1), 47–51. [3]



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 10