Difference between revisions of "Part:BBa K143050"

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P43-gsiB can be used in the context of a '''Polymerases per second''' (PoPS) output generator.
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P43-gsiB can be used in the context of a '''Ribosomes per second''' (RiPS) output generator.
  
  

Latest revision as of 15:06, 6 October 2008

Promoter 43 and RBS gsiB for B. subtilis


Constitutive promoter 43(BBa_K143013) coupled to the strong Ribosome Binding Site gsiB(BBa_K143020) from B. subtilis.


P43-gsiB can be used in the context of a Ribosomes per second (RiPS) output generator.


To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix1. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.


Sequence and Features



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

<biblio>

  1. 1 https://parts.igem.org/Assembly:RBS-CDS_issues

</biblio>