Difference between revisions of "Part:BBa K3038003:Design"

(→‎References)
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===Design Notes===
 
===Design Notes===
 
The sequence has been designed to meet the RFC10 standard.  
 
The sequence has been designed to meet the RFC10 standard.  
−
GC content has been optimized
 
−
a Flag Tag has been added in the C term part of the sequence
 
  
 +
GC content has been optimized.
  
 +
a Flag Tag has been added in the C term part of the sequence.
  
 
===Source===
 
===Source===

Revision as of 12:39, 16 October 2019


FadM C-ter Flag-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence has been designed to meet the RFC10 standard.

GC content has been optimized.

a Flag Tag has been added in the C term part of the sequence.

Source

Sequence synthesis by IDT

References

Engineering of Bacterial Methyl Ketone Synthesis for Biofuels. Ee-Been Goh,a,c Edward E. K. Baidoo,a,c Jay D. Keasling,a,c,d and Harry R. Beller. Appl Environ Microbiol. 2012 Jan; 78(1): 70–80. doi: 10.1128/AEM.06785-11. PMCID: PMC3255637. PMID: 22038610