Difference between revisions of "Part:BBa K1758100:Design"

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===Source===
 
===Source===
  
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This part was created by applying Gibson primers with suitable overhangson pSB1C3.
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This part was created by applying Gibson primers with suitable overhangs on pSB1C3.
  
 
===References===
 
===References===

Latest revision as of 12:36, 16 October 2019


Translation enhancing 5-UTR containing g10-L RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We derived this sequence from the literature, searching for the best suited enhancing sequence for in vitro translation. Our main literature sources were [http://www.ncbi.nlm.nih.gov/pubmed/2676996 Olins et al. 1989], [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3161345/ Shin and Noireaux 2010], [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3128707/ Zawada et al. 2011], [http://www.ncbi.nlm.nih.gov/pubmed/23654270 Lentini et al. 2013] and [http://www.ncbi.nlm.nih.gov/pubmed/23927491 Takahashi et al. 2013].

Source

This part was created by applying Gibson primers with suitable overhangs on pSB1C3.

References