Difference between revisions of "Part:BBa K3096053"
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==References==
 
==References==
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#'''Xia, Peng-Fei, et al'''. "Synthetic genetic circuits for programmable biological functionalities." ''Biotechnology advances'' ('''2019''').
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#'''Terns, Michael P., and Rebecca M. Terns'''. "CRISPR-based adaptive immune systems." ''Current opinion in microbiology'' 14.3 ('''2011'''): 321-327.
 
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K3096053 parameters</partinfo>
 
<partinfo>BBa_K3096053 parameters</partinfo>
 
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Latest revision as of 11:39, 16 October 2019

CRISPR Array targeting the Plasmid DNA of the Exendin-4 expression system controlled by Lsr

The CRISPR array sequence needed to guide the Cas3 nuclease to its cut sites on the plasmid with the glucose dependent Exendin-4 expression system under the control of the LsrR promoter. When LsrR is present the array is not transcribed and the CRISPR/Cas3 System lacks its gRNA and is inactive. Combined with the fatty acid responsive repression system (BBa_K3096046) the expression of the array can be controlled.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

  1. Xia, Peng-Fei, et al. "Synthetic genetic circuits for programmable biological functionalities." Biotechnology advances (2019).
  2. Terns, Michael P., and Rebecca M. Terns. "CRISPR-based adaptive immune systems." Current opinion in microbiology 14.3 (2011): 321-327.