Difference between revisions of "Part:BBa K2984031"

 
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<partinfo>BBa_K2984031 short</partinfo>
 
<partinfo>BBa_K2984031 short</partinfo>
  
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This part is composed of the PsaD promoter (BBa_K2984008), the GLE secretion signal (BBa_K2984001), the PETase enzyme (), a YFP mVenus marker (BBa_K2984017), and the Rbcs2 terminator (BBa_K2984018). The part can be used to express and secrete the PETase enzyme in C. reinhardtii. The YFP-mVenus tag can be used to detect the expression and secretion of the PETase.  
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This part is composed of the PsaD promoter (BBa_K2984008), the ARS secretion signal (BBa_K2984000), the PETase enzyme (), a YFP mVenus marker (BBa_K2984017), and the Rbcs2 terminator (BBa_K2984018). The part can be used to express and secrete the PETase enzyme in C. reinhardtii. The YFP-mVenus tag can be used to detect the expression and secretion of the PETase.  
  
 
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Revision as of 11:16, 16 October 2019


L1c-PsaD-ARS-PETase-YFP-RBCS2

This part is composed of the PsaD promoter (BBa_K2984008), the ARS secretion signal (BBa_K2984000), the PETase enzyme (), a YFP mVenus marker (BBa_K2984017), and the Rbcs2 terminator (BBa_K2984018). The part can be used to express and secrete the PETase enzyme in C. reinhardtii. The YFP-mVenus tag can be used to detect the expression and secretion of the PETase.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1979
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1308
    Illegal PstI site found at 1979
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
    Illegal XhoI site found at 1032
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1979
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1979
    Illegal NgoMIV site found at 2456
  • 1000
    COMPATIBLE WITH RFC[1000]