Difference between revisions of "Part:BBa K2996007"
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<partinfo>BBa_K2996007short</partinfo>
 
Expression of the CRISPR adaptation proteins Cas1 and Cas2 promotes unidirectional integration of 33–base pair DNA spacers into our designed RSRL array.
 
Expression of the CRISPR adaptation proteins Cas1 and Cas2 promotes unidirectional integration of 33–base pair DNA spacers into our designed RSRL array.
  
 
Addition of tetracycline will activate the expression of cas1-cas2 complex, thus accomplishing information storage.
 
Addition of tetracycline will activate the expression of cas1-cas2 complex, thus accomplishing information storage.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2996007 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K2996007 parameters</partinfo>
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Revision as of 10:43, 16 October 2019


No part name specified with partinfo tag. Expression of the CRISPR adaptation proteins Cas1 and Cas2 promotes unidirectional integration of 33–base pair DNA spacers into our designed RSRL array.

Addition of tetracycline will activate the expression of cas1-cas2 complex, thus accomplishing information storage.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 170
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 891
  • 1000
    COMPATIBLE WITH RFC[1000]