Difference between revisions of "Part:BBa K3102031:Design"

Latest revision as of 10:41, 16 October 2019

T7-RBS-ACS-Ter

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 408
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 837
1000
COMPATIBLE WITH RFC[1000]

Design Notes

We mutated the ACS sequence with a Cytosine into Thymine at the 1563bp position (C1563T) because of the Esp3I enzyme that we used for our Golden Gate Assembly (GGA) construct. (BBa_K3102021)

Source

E.coli, sequence found through Biocyc https://biocyc.org/gene?orgid=ECOLI&id=EG11448

Promoter (BBa_I719005) , RBS (BBa_B0034) and Terminator (BBa_B0015) are from the iGEM Registry.

References

Brown TD. et al., The enzymic interconversion of acetate and acetyl-coenzyme A in Escherichia coli, J Gen Microbiol. 1977 Oct;102(2):327-36.

Kumari S. et al., Cloning, characterization, and functional expression of acs, the gene which encodes acetyl coenzyme A synthetase in Escherichia coli, J Bacteriol. 1995 May;177(10):2878-86.

@@ Line 8: / Line 8: @@
 ===Design Notes===
-We mutated a Cytosine into Thymine at the 1563bp position (C1563T) because of the Esp3I enzyme that we used for our Golden Gate Assembly (GGA) construct. (e.g BBa_K3102021)
+We mutated the ACS sequence with a Cytosine into Thymine at the 1563bp position (C1563T) because of the Esp3I enzyme that we used for our Golden Gate Assembly (GGA) construct. (BBa_K3102021)
 ===Source===