Difference between revisions of "Part:BBa K2800027"
Line 19: Line 19:
 
<p style="text-align: center;padding:0 0;">Fig.1 The principle of Tet and TetR/TetO system.   
 
<p style="text-align: center;padding:0 0;">Fig.1 The principle of Tet and TetR/TetO system.   
 
</p>
 
</p>
 +
<h1><b>Characterize</b></h1>
 +
<img src="https://static.igem.org/mediawiki/parts/e/e2/T--HUBU_WUHAN--mechanism_of_TC.png" alt="" style="text-align: center;width:60%;padding:0 0;">
 +
<p style="text-align: center;padding:0 0;">Fig.2 The mechanism of Tc.
 +
</p>
 +
<p>
 +
  
 
</html>
 
</html>

Revision as of 10:22, 16 October 2019


Ptet promoter

-

Usage and Biology

Biology:

    This part is inducible promoter Ptet which is induced by tetracycline. This is the basic principle of Tet induced expression system, it is to regulate the conformation of proteins by inducer such as Tet, thus controlling the expression of target proteins. When there is no tetracycline, TetR will bind to TetO to block downstream expression of resistant genes. When tetracycline is added, it will bind to TetR and change its conformation, then TetR is separated from TetO, thereby relieving the inhibition of resistance genes.

Fig.1 The principle of Tet and TetR/TetO system.

Characterize

Fig.2 The mechanism of Tc.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]