Difference between revisions of "Part:BBa K3239011"

(→‎Usage and Biology)
(→‎Usage and Biology)
Line 9: Line 9:
 
This construct should not only exhibit a combined effect of its two subparts but also the more complex interactions among these two constructs. Figure 1 illustrates the presumed complex regulation of pAOX1.  
 
This construct should not only exhibit a combined effect of its two subparts but also the more complex interactions among these two constructs. Figure 1 illustrates the presumed complex regulation of pAOX1.  
 
[[File:pGMP1-pAPM1-pAOX1-GFP.png|400px|thumb|Figure 1. Illustration of the pGMP-pAPM1-pAOX1-GFP construct (for in trans regulations among homogenous transcription factors and the homogenous AOX1, see the pAOX1-GFP construct)]]
 
[[File:pGMP1-pAPM1-pAOX1-GFP.png|400px|thumb|Figure 1. Illustration of the pGMP-pAPM1-pAOX1-GFP construct (for in trans regulations among homogenous transcription factors and the homogenous AOX1, see the pAOX1-GFP construct)]]
 +
 +
 +
 +
 +
 +
 +
 +
  
  

Revision as of 10:08, 16 October 2019


pMIT1-PRM1-pPRM1-MIT1

A combination of the pMIT1-PRM1 and the pPRM1-MIT1 constructs.


Usage and Biology

This construct should not only exhibit a combined effect of its two subparts but also the more complex interactions among these two constructs. Figure 1 illustrates the presumed complex regulation of pAOX1.

Figure 1. Illustration of the pGMP-pAPM1-pAOX1-GFP construct (for in trans regulations among homogenous transcription factors and the homogenous AOX1, see the pAOX1-GFP construct)










Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2034
    Illegal SpeI site found at 793
    Illegal PstI site found at 2556
    Illegal PstI site found at 6970
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2034
    Illegal NheI site found at 3815
    Illegal SpeI site found at 793
    Illegal PstI site found at 2556
    Illegal PstI site found at 6970
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2034
    Illegal BglII site found at 1387
    Illegal BglII site found at 4268
    Illegal BglII site found at 7428
    Illegal BamHI site found at 2917
    Illegal BamHI site found at 3836
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2034
    Illegal SpeI site found at 793
    Illegal PstI site found at 2556
    Illegal PstI site found at 6970
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2034
    Illegal SpeI site found at 793
    Illegal PstI site found at 2556
    Illegal PstI site found at 6970
  • 1000
    COMPATIBLE WITH RFC[1000]