Difference between revisions of "Part:BBa K3239010:Design"

 
(→‎Source)
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
P.pastoris GS115
+
P.pastoris GS115 and https://www.ncbi.nlm.nih.gov/nuccore/82569479
 +
and
 
BBa_K3239000
 
BBa_K3239000
 
BBa_K3239004
 
BBa_K3239004
  
 
===References===
 
===References===

Revision as of 09:55, 16 October 2019


pPRM1-MIT1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2995
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2995
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 293
    Illegal BglII site found at 3453
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2995
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2995
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Parts were fused via Gibson assembly, hence there were no scars.


Source

P.pastoris GS115 and https://www.ncbi.nlm.nih.gov/nuccore/82569479 and BBa_K3239000 BBa_K3239004

References