Difference between revisions of "Part:BBa K3089021"

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<span class='h3bb'>Sequence and Features</span>
 
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===Functional Parameters===
 
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Revision as of 09:42, 16 October 2019


No part name specified with partinfo tag.

This composite part is meant to express csgA-linker-mfp5 fusion genes under T7 promoter and purify CsgA-linker-Mfp5 recombinant proteins by His-tag affinity purification. CsgA is an amyloid-like protein encoded on the genome of E.coli MG1655 providing mechanical cohesive strength and Mfp5(BBa_K1583002)is a mussel foot protein from Mytilus galloprovincialis responsible for interface adhesion. This recombinant protein would self-assemble into fibrous bundles or films with adhesivepropertiesby displaying the mussel adhesion domains on the surface of amyloid scaffolds, which would be a promising new generation of bio-inspired adhesives for a wide range of applications.

This part was designed based on the paper "Strong underwater adhesives made by self-assembling multi-protein nanofibres" (Zhong et al, 2014.)

Sequence and Features No part name specified with partinfo tag.