Difference between revisions of "Part:BBa K3102045:Design"

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===Design Notes===
 
===Design Notes===
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We mutated the MtrC with a Guanine into Cysteine and a Guanine into Adenine at the 1838bp and 1841bp position (G1838C & G1841A), respectively, because of the PstI enzyme.
  
 
===Source===
 
===Source===

Revision as of 09:03, 16 October 2019


Synthesis and externalization of lactate (DLD-II Operon, MtrC)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4365
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4949


Design Notes

We mutated the MtrC with a Guanine into Cysteine and a Guanine into Adenine at the 1838bp and 1841bp position (G1838C & G1841A), respectively, because of the PstI enzyme.

Source

DLD-II: sequence comes from Shewanella Oneidensis was found through Biocyc: https://biocyc.org/gene?orgid=SONE211586&id=G1GMP-1405-MONOMER#tab=TU

MtrC: sequence comes from Shewanella Oneidensis was found through Biocyc: https://biocyc.org/gene?orgid=SONE211586&id=G1GMP-1627

Promoter (BBa_I719005), RBS (BBa_B0034) and Terminator (BBa_B0015) are from the iGEM Registry.

References

Kouzuma A, Kasai T, Hirose A, Watanabe K. "Catabolic and regulatory systems in shewanella oneidensis MR-1 involved in electricity generation in microbial fuel cells". Front Microbiol. 2015;6(JUN):1–11.