Difference between revisions of "Part:BBa K3102047:Design"

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===References===
 
===References===
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Bacher A. et al., "Riboflavin synthases of Bacillus subtilis. Purification and properties." Biol. Chem. 255:632-637(1980)
 +
 +
Eberhardt S.M.R. et al., J"Cloning, sequencing, mapping and hyperexpression of the ribC gene coding for riboflavin synthase of Escherichia coli." . Biochem. 242:712-719(1996)
 +
 +
Lin Z, et al., "Metabolic engineering of Escherichia coli for the production of riboflavin". Microb Cell Fact. 2014;13(1):1–12.
 +
 +
Zhenquan Lin et al., “Metabolic engineering of Escherichia coli for the production of riboflavin," Microbial Cell Factories, 2014, 13:104.

Latest revision as of 08:39, 16 October 2019


Electron transfer improvement (Riboflavin, RibF)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 354
    Illegal PstI site found at 3318
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 354
    Illegal PstI site found at 3318
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1013
    Illegal BglII site found at 2110
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 354
    Illegal PstI site found at 3318
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 354
    Illegal PstI site found at 3318
    Illegal AgeI site found at 201
    Illegal AgeI site found at 903
    Illegal AgeI site found at 945
    Illegal AgeI site found at 1488
    Illegal AgeI site found at 1863
    Illegal AgeI site found at 3819
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

RibA : E.coli, sequence found through Biocyc https://biocyc.org/gene?orgid=ECOLI&id=EG11331

RibB: E.coli, sequence found through Biocyc https://biocyc.org/gene?orgid=ECOLI&id=EG10465

RibD: E.coli, sequence found through Biocyc https://biocyc.org/gene?orgid=ECOLI&id=EG11321

RibE: E.coli, sequence found through Biocyc https://biocyc.org/gene?orgid=ECOLI&id=EG11322

RibC: E.coli, sequence found through Biocyc https://biocyc.org/gene?orgid=ECOLI&id=EG11406

RibF: E.coli, sequence found through Biocyc https://biocyc.org/gene?orgid=ECOLI&id=EG11079


Promoter (BBa_I719005), RBS (BBa_B0034) and Terminator (BBa_B0015) are from the iGEM Registry.

References

Bacher A. et al., "Riboflavin synthases of Bacillus subtilis. Purification and properties." Biol. Chem. 255:632-637(1980)

Eberhardt S.M.R. et al., J"Cloning, sequencing, mapping and hyperexpression of the ribC gene coding for riboflavin synthase of Escherichia coli." . Biochem. 242:712-719(1996)

Lin Z, et al., "Metabolic engineering of Escherichia coli for the production of riboflavin". Microb Cell Fact. 2014;13(1):1–12.

Zhenquan Lin et al., “Metabolic engineering of Escherichia coli for the production of riboflavin," Microbial Cell Factories, 2014, 13:104.