Difference between revisions of "Part:BBa K3102042"

 
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<partinfo>BBa_K3102042 short</partinfo>
 
<partinfo>BBa_K3102042 short</partinfo>
  
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This cellulose acetate degradation (ACE, EG1, TEV, HlyA) composite part composes of acetate esterase (CAE):BBa_K3102023, endo-1,4-B-glucanase I (EG1):BBa_K3102024 and TEV protease (TEV):BBa_K3102025 permit to secrete these enzymes directly in the extracellular medium using E. coli type I secretion system.  
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This cellulose acetate degradation (ACE, EG1, TEV, HlyA) composite part is  composed of acetate esterase (CAE):BBa_K3102023, endo-1,4-B-glucanase I (EG1):BBa_K3102024, and TEV protease (TEV):BBa_K3102025. This part allows the secretion of these enzymes directly in the extracellular medium using E. coli type I secretion system.
  
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We used an inducible promoter T7 BBa_I719005 in order to control CAE, EG1 and TEV production thanks to IPTG induction
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We used an inducible promoter T7 BBa_I719005 in order to control CAE, EG1 and TEV production thanks to IPTG induction.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 08:30, 16 October 2019


Cellulose Acetate degradation (CAE, EG1, Tev, HlyA)

This cellulose acetate degradation (ACE, EG1, TEV, HlyA) composite part is composed of acetate esterase (CAE):BBa_K3102023, endo-1,4-B-glucanase I (EG1):BBa_K3102024, and TEV protease (TEV):BBa_K3102025. This part allows the secretion of these enzymes directly in the extracellular medium using E. coli type I secretion system.

We used an inducible promoter T7 BBa_I719005 in order to control CAE, EG1 and TEV production thanks to IPTG induction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2958
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 124
    Illegal NgoMIV site found at 1234
    Illegal NgoMIV site found at 4120
    Illegal NgoMIV site found at 4426
    Illegal AgeI site found at 254
    Illegal AgeI site found at 341
    Illegal AgeI site found at 479
    Illegal AgeI site found at 662
    Illegal AgeI site found at 710
    Illegal AgeI site found at 878
    Illegal AgeI site found at 1091
  • 1000
    COMPATIBLE WITH RFC[1000]