Difference between revisions of "Part:BBa K143062:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K143062 short</partinfo>
 
<partinfo>BBa_K143062 short</partinfo>
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<partinfo>BBa_K143062 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K143062 SequenceAndFeatures</partinfo>
 
  
 
===Design Notes===
 
===Design Notes===
 
LacI was identified from the pDR111 ''B. subtilis'' integration vector. The double terminator is the most commonly used registry terminator.
 
LacI was identified from the pDR111 ''B. subtilis'' integration vector. The double terminator is the most commonly used registry terminator.
 
 
  
 
===Source===
 
===Source===
  
 
LacI was produced by PCR cloning using Pfu form the ''B. subtilis'' integration vector and cloned into a BioBrick with the registry double terminator.
 
LacI was produced by PCR cloning using Pfu form the ''B. subtilis'' integration vector and cloned into a BioBrick with the registry double terminator.
 
===References===
 

Revision as of 15:01, 1 October 2008

LacI repressor protein - Terminator



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

LacI was identified from the pDR111 B. subtilis integration vector. The double terminator is the most commonly used registry terminator.

Source

LacI was produced by PCR cloning using Pfu form the B. subtilis integration vector and cloned into a BioBrick with the registry double terminator.