Difference between revisions of "Part:BBa K143062"

Revision as of 15:00, 1 October 2008

LacI repressor protein - Terminator

LacI transcriptional repressor protein (BBa_K143033) coupled to the double terminator (BBa_B0015.

The LacI does not possess a LVA degradation tag and gas a short (3 amino acid) N-terminal deletion consistent with LacI used in conjunction with B. subtilis.

LacI can be used in conjunction with the lac operon promoter (BBa_K143015), where the LacI will act as a receiver for an IPTG input to result in a Polymerases per second (PoPS) output.

The double terminator is the most commonly used terminator and is a combination of parts BBa_B0010 and BBa_B0012.

The double terminator allows the LacI to be easily incorporated into a closed transcriptional unit.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K143062 short</partinfo>
 LacI transcriptional repressor protein (<bbpart>BBa_K143033</bbpart>) coupled to the double terminator (<bbpart>BBa_B0015</bbpart>.
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 The double terminator allows the LacI to be easily incorporated into a closed transcriptional unit.
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-<span class='h3bb'>Sequence and Features</span>
+<span class='h3bb'><big>'''Sequence and Features'''</big></span>
 <partinfo>BBa_K143062 SequenceAndFeatures</partinfo>