Difference between revisions of "Part:BBa K2912011"
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Revision as of 04:55, 16 October 2019
M. micrantha_leaves_ Unigene0029128
This is a single-stranded DNA that can be cyclized by T7 promoter, since it has two sites complementarty to T7 promoter. It can transcribe RNA interference (RNAi) molecule by rolling circle transcription, which can silence the gene encoding chlorophyll A-B binding family protein AB80 of M. micrantha, through which we can block this essential matabolic gene expression to kill such invasive weed.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
SZU-China 2019 iGEM team
SZU-China 2019 iGEM team decides to synthesize the Micrancide, an RNAi-based herbicide for M. micrantha, to remove the weed by silencing the essential metabolic gene of it through RNA interference (RNAi) technology.
RNA interference technology
Short-interfering RNAs suppress gene expression through a highly regulated enzyme-mediated process called RNA interference (RNAi). RNAi is a biological process in which RNA molecules inhibit gene expression or translation, by neutralizing targeted mRNA molecules [3]. It involves multiple RNA-protein interactions characterized by four major steps:
- Assembly of siRNA with the RNA-induced silencing complex (RISC)
