Difference between revisions of "Part:BBa K2927006"
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The particular structure of guide RNA makes it has an affinity with Cas12a protein. The feature lets crRNA which is the sequence behind guide RNA, the sequence is used to recognize its target double-stranded DNA.
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The CRISPR/Case system uses guide RNA (gRNA) and crRNAs to recognize their targets. The gRNA sequence binds to Cas12a protein, which promotes crRNA to recognize its target double-stranded DNA through sequence complementation.
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===Usage and Biology===
 
===Usage and Biology===

Revision as of 04:54, 16 October 2019


guide RNA of LbCas12a

The CRISPR/Case system uses guide RNA (gRNA) and crRNAs to recognize their targets. The gRNA sequence binds to Cas12a protein, which promotes crRNA to recognize its target double-stranded DNA through sequence complementation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]