Difference between revisions of "Part:BBa K143050"
m |
m |
||
| Line 5: | Line 5: | ||
Constitutive promoter 43(<bbpart>BBa_K143013</bbpart>) coupled to the strong Ribosome Binding Site gsiB(<bbpart>BBa_K143020</bbpart>) from ''B. subtilis''. | Constitutive promoter 43(<bbpart>BBa_K143013</bbpart>) coupled to the strong Ribosome Binding Site gsiB(<bbpart>BBa_K143020</bbpart>) from ''B. subtilis''. | ||
| − | P43-gsiB can be used in the context of a | + | |
| + | P43-gsiB can be used in the context of a '''Polymerases per second''' (PoPS) output generator. | ||
| + | |||
| + | |||
| + | '''To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix<cite>1</cite>. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.''' | ||
| Line 16: | Line 20: | ||
<partinfo>BBa_K143050 SequenceAndFeatures</partinfo> | <partinfo>BBa_K143050 SequenceAndFeatures</partinfo> | ||
| + | |||
| + | |||
| + | ===References=== | ||
| + | <biblio> | ||
| + | #1 https://parts.igem.org/Assembly:RBS-CDS_issues | ||
| + | </biblio> | ||
Revision as of 11:59, 30 September 2008
Promoter 43 and RBS gsiB for B. subtilis
Constitutive promoter 43(BBa_K143013) coupled to the strong Ribosome Binding Site gsiB(BBa_K143020) from B. subtilis.
P43-gsiB can be used in the context of a Polymerases per second (PoPS) output generator.
To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix1. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
<biblio>
</biblio>