Difference between revisions of "Part:BBa K3317051:Design"
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===References===
 
===References===
  
−
CIDEB-UANL_Mexico. (2014). Capture module. Accessed 24 April 2018, <http://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_capture>
+
<p>CIDEB-UANL_Mexico. (2014). Capture module. Accessed 24 April 2018, <http://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_capture></p>
−
Huang, L. (2010). Growth kinetics of Escherichia coli O157:H7 in mechanically-tenderized beef. International Journal of Food Microbiology. No. 140, pp. 40–48.
+
<p>Huang, L. (2010). Growth kinetics of Escherichia coli O157:H7 in mechanically-tenderized beef. International Journal of Food Microbiology. No. 140, pp. 40–48.</p>
−
Krulwich, T.& Ivey, M. (1994) Sodium ion binding proteins. Accessed 24 April 2018, <http://www.google.com.mx/patents/US5346815>.
+
<p>Krulwich, T.& Ivey, M. (1994) Sodium ion binding proteins. Accessed 24 April 2018, <http://www.google.com.mx/patents/US5346815>.</p>

Revision as of 22:57, 15 October 2019


NhaS with an RFP reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 849
    Illegal AgeI site found at 961
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The promoter was modified for its constitutive production and its characterization, adding the BBa_J23119 part at the beginning of the sequence and replacing its UV-activated promoter from the original BBa_K1255002 part.


Source

The original sequence was described by Krulwich in 1994 as a hypothetical gene. However, this part has been optimized from the BBa_K1255002 part registered by the CIDEB-UANL_Mexico team in 2014.

References

CIDEB-UANL_Mexico. (2014). Capture module. Accessed 24 April 2018, <http://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_capture>

Huang, L. (2010). Growth kinetics of Escherichia coli O157:H7 in mechanically-tenderized beef. International Journal of Food Microbiology. No. 140, pp. 40–48.

Krulwich, T.& Ivey, M. (1994) Sodium ion binding proteins. Accessed 24 April 2018, <http://www.google.com.mx/patents/US5346815>.