Difference between revisions of "Part:BBa K2984013:Design"
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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K2984025 short</partinfo>
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<partinfo>BBa_K2984009 short</partinfo>
  
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<partinfo>BBa_K2984025 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K2984009 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
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Codon syntax fits <i>C. reinhardtii</i>. This was designed as a B3-B4 part according to the MoClo standard of Patron et al. (2015).
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Codon syntax fits <i>C. reinhardtii</i>. This was designed as a A1-A3 part according to the MoClo standard of Patron et al. (2015).
  
  
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===Source===
 
===Source===
  
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Loera-Quezada, M. M., Leyva-González, M. A., Velázquez-Juárez, G., Sanchez-Calderón, L., Do Nascimento, M., López-Arredondo, D., & Herrera-Estrella, L. (2016). A novel genetic engineering platform for the effective management of biological contaminants for the production of microalgae. Plant Biotechnol J, 14(10), 2066-2076.
+
Schroda, M., et al. (2002). "Sequence elements within an HSP70 promoter counteract transcriptional transgene silencing in Chlamydomonas." Plant J 31(4): 445-455.
  
 
===References===
 
===References===
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Loera-Quezada, M. M., Leyva-Gonzalez, M. A., Lopez-Arredondo, D., & Herrera-Estrella, L. (2015). Phosphite cannot be used as a phosphorus source but is non-toxic for microalgae. Plant Sci, 231, 124-130. doi:10.1016/j.plantsci.2014.11.015.
+
 
−
Loera-Quezada, M. M., Leyva-González, M. A., Velázquez-Juárez, G., Sanchez-Calderón, L., Do Nascimento, M., López-Arredondo, D., & Herrera-Estrella, L. (2016). A novel genetic engineering platform for the effective management of biological contaminants for the production of microalgae. Plant Biotechnol J, 14(10), 2066-2076.
+
Schroda, M., et al. (2002). "Sequence elements within an HSP70 promoter counteract transcriptional transgene silencing in Chlamydomonas." Plant J 31(4): 445-455.
−
Patron, N. J., Orzaez, D., Marillonnet, S., Warzecha, H., Matthewman, C., Youles, M., . . . Haseloff, J. (2015). Standards for plant synthetic biology: a common syntax for exchange of DNA parts. New Phytologist, 208(1), 13-19.
+
Patron, N. J., Orzaez, D., Marillonnet, S., Warzecha, H., Matthewman, C., Youles, M., . . . Haseloff, J. (2015). Standards for plant synthetic biology: a common syntax for exchange of DNA parts. New Phytologist, 208(1), 13-19. Retrieved from https://nph.onlinelibrary.wiley.com/doi/abs/10.1111/nph.13532.
 +
Schroda, M., et al. (2000). "The HSP70A promoter as a tool for the improved expression of transgenes in Chlamydomonas." Plant J 21(2): 121-131.

Revision as of 21:51, 15 October 2019

AR Promoter A1-A3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 282
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 685
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon syntax fits C. reinhardtii. This was designed as a A1-A3 part according to the MoClo standard of Patron et al. (2015).


Source

Schroda, M., et al. (2002). "Sequence elements within an HSP70 promoter counteract transcriptional transgene silencing in Chlamydomonas." Plant J 31(4): 445-455.

References

Schroda, M., et al. (2002). "Sequence elements within an HSP70 promoter counteract transcriptional transgene silencing in Chlamydomonas." Plant J 31(4): 445-455. Patron, N. J., Orzaez, D., Marillonnet, S., Warzecha, H., Matthewman, C., Youles, M., . . . Haseloff, J. (2015). Standards for plant synthetic biology: a common syntax for exchange of DNA parts. New Phytologist, 208(1), 13-19. Retrieved from https://nph.onlinelibrary.wiley.com/doi/abs/10.1111/nph.13532. Schroda, M., et al. (2000). "The HSP70A promoter as a tool for the improved expression of transgenes in Chlamydomonas." Plant J 21(2): 121-131.