Difference between revisions of "Part:BBa K2996709"

Revision as of 17:01, 15 October 2019

LuxI downstream of activation unit containing lure

LuxI is put downstream of BBa_K2996705, as a reporter, which is a synthetase for acyl-homoserine lactones (AHL). dcas9 binding to lure means the occurrence of off-target incidence. RpoA, which is linked to dcas9, will recruits RNA polymerase and the gene downstream of the promotor will be activated. We develop a way to record off-target incidence, with LuxI gene under the control of promotor J23117. Increased expression of luxI enzyme will synthesis enough AHL molecules, which can penetrate to the bacteria capable of information storage.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 52
Illegal NheI site found at 75
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 743
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 39
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2996709 short</partinfo>
-well
+LuxI is put downstream of BBa_K2996705, as a reporter, which is a synthetase for acyl-homoserine lactones (AHL).
+dcas9 binding to lure means the occurrence of off-target incidence. RpoA, which is linked to dcas9, will recruits RNA polymerase and the gene downstream of the promotor will be activated.
+We develop a way to record off-target incidence, with LuxI gene under the control of promotor J23117. Increased expression of luxI enzyme will synthesis enough AHL molecules, which can penetrate to the bacteria capable of information storage.
 <!-- Add more about the biology of this part here