Difference between revisions of "Part:BBa K3040506"

 
 
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pFadD promoter is one of the regulator in the enzymes of fatty acid biosynthesis in E. coli. It is composed of two fadR recognition sites, and one CRP binding site. The fatty acid metabolism system of E. coli is consist of many parts, fadD, fadL, fadR, fadA, etc. Interestingly, each enzyme in this family has its own different sequence of fadR recognition site in its promoter, which makes these promoter having different strength, yet can still be interchangeable for us to apply in our system.
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Based on this fact, we tried pFadD promoter to take place of the weak pFadBA promoter. With two fadR recognition sites native in pFadD, we assume a better result in the sensitivity and a lower leakage in our pFadD promoter.
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K3040005 parameters</partinfo>
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<partinfo>BBa_K3040506 parameters</partinfo>
 
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Latest revision as of 16:38, 15 October 2019


pFadBA_NTHU

pFadD promoter is one of the regulator in the enzymes of fatty acid biosynthesis in E. coli. It is composed of two fadR recognition sites, and one CRP binding site. The fatty acid metabolism system of E. coli is consist of many parts, fadD, fadL, fadR, fadA, etc. Interestingly, each enzyme in this family has its own different sequence of fadR recognition site in its promoter, which makes these promoter having different strength, yet can still be interchangeable for us to apply in our system. Based on this fact, we tried pFadD promoter to take place of the weak pFadBA promoter. With two fadR recognition sites native in pFadD, we assume a better result in the sensitivity and a lower leakage in our pFadD promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 65
    Illegal suffix found in sequence at 271
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 65
    Illegal SpeI site found at 272
    Illegal PstI site found at 286
    Illegal NotI site found at 71
    Illegal NotI site found at 279
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 65
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 65
    Illegal suffix found in sequence at 272
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 65
    Illegal XbaI site found at 80
    Illegal SpeI site found at 272
    Illegal PstI site found at 286
  • 1000
    COMPATIBLE WITH RFC[1000]