Difference between revisions of "Part:BBa K3279006"
Line 8: Line 8:
 
===Usage and Biology===
 
===Usage and Biology===
  
−
<!-- -->
 
−
<span class='h3bb'>Sequence and Features</span>
 
−
<partinfo>BBa_K3279006 SequenceAndFeatures</partinfo>
 
−
 
−
===Characterization from iGEM19_CAU_China===
 
 
With INP’s impressive ability, we fused the cellulases with INP-N to accomplish the purpose: cellulose degradation on the surface of bacteria. We linked the cellulases (cex and cenA seperately) into a pET30a(+) backbone, then transformed this plasmid into BL21. We induced these recombinant E.coli strains overnight under the condition of 16℃ 0.08 mM. See Fig.1[1].
 
With INP’s impressive ability, we fused the cellulases with INP-N to accomplish the purpose: cellulose degradation on the surface of bacteria. We linked the cellulases (cex and cenA seperately) into a pET30a(+) backbone, then transformed this plasmid into BL21. We induced these recombinant E.coli strains overnight under the condition of 16℃ 0.08 mM. See Fig.1[1].
  
Line 18: Line 13:
  
 
Then the effect of fusion on enzyme activity was detected by measuring the cellulose degradation ability using CMC-Na as substrate. See Fig.3[1].
 
Then the effect of fusion on enzyme activity was detected by measuring the cellulose degradation ability using CMC-Na as substrate. See Fig.3[1].
 +
 +
<!-- -->
 +
<span class='h3bb'>Sequence and Features</span>
 +
<partinfo>BBa_K3279006 SequenceAndFeatures</partinfo>
 +
 +
  
  

Revision as of 16:25, 15 October 2019


INP-N ( N-terminus of Ice-nucleation protein ) from Pseudomonas syringae

Ice-nucleation protein (INP) is a secreted outer membrane protein, which is widely distributed in Pseudomonas syringae, Pseudomonas fluorescens and other Gram-negative bacteria. Compared with other surface carrier proteins, ice-nucleation protein has the advantage of stably expressing heterogenous proteins and displaying proteins with larger molecular weight. This part includes the sequence of INP-N (N terminus of Ice-nucleation protein), which is much shorter than INP and INP-NC. To allow this part fusion with other protein, we added an additional linker sequence at the 3'.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 333
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 75
    Illegal NgoMIV site found at 408
  • 1000
    COMPATIBLE WITH RFC[1000]