Difference between revisions of "Part:BBa J52008"
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We transferred the plasmid with only carrying hRluc and stimulated them with different concentration of sugar. With the purpose of detecting the correlation between the expression of hRluc and the stimulation of the sugar’s concentration, we tracked the cells and used the double luciferase kit to test their correlation. And the result showed below. | We transferred the plasmid with only carrying hRluc and stimulated them with different concentration of sugar. With the purpose of detecting the correlation between the expression of hRluc and the stimulation of the sugar’s concentration, we tracked the cells and used the double luciferase kit to test their correlation. And the result showed below. | ||
| − | + | https://2019.igem.org/wiki/images/b/be/T--NUDT_CHINA--result_of_SV40_promoter.png | |
Revision as of 15:40, 15 October 2019
luciferase: luciferin 2-monooxygenase from Renilla reniformis (EC 1.13.12.5; SwissProt:: P27652)
Rluc is a protein called Renilla luciferase and it emits light when the right substrate is added. That is why it can be used as a reporter to track other proteins or to monitor the activity of a promoter.
Usage and Biology
This part contains a Eukaryotic ribosome binding site and non-standard prefix and suffix. See the sequence first.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 547
experiment
We transferred the plasmid with only carrying hRluc and stimulated them with different concentration of sugar. With the purpose of detecting the correlation between the expression of hRluc and the stimulation of the sugar’s concentration, we tracked the cells and used the double luciferase kit to test their correlation. And the result showed below.
