Difference between revisions of "Part:BBa K3279007"

Revision as of 15:30, 15 October 2019

β-Glucosidase from Streptomyces coelicolor

β-Glucosidase obtained from various sources has been used in many areas, such as the enzymatic saccharification of cellulosic materials, the liberation of flavor compounds in fruit juices and wines, and so on. In cellulose hydrolysis, β-glucosidase is used to degrade cello-oligosaccharides to glucose[1]. We used this part to accomplish the cellulose degradation.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 287
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 427
Illegal NgoMIV site found at 1103
Illegal AgeI site found at 184
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 687
Illegal BsaI.rc site found at 946

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 &#946;-Glucosidase obtained from various sources has been used in many areas, such as the enzymatic saccharification of cellulosic materials, the liberation of flavor compounds in fruit juices and wines, and so on. In cellulose hydrolysis, &#946;-glucosidase is used to degrade cello-oligosaccharides to glucose[1]. We used this part to accomplish the cellulose degradation.
-====References====
-[1]Gu, M. Z., Wang, J. C., Liu, W. B., Zhou, Y. & Ye, B. C. Expression and displaying of beta-glucosidase from Streptomyces coelicolor A3 in Escherichia coli. Applied biochemistry and biotechnology 170, 1713-1723, doi:10.1007/s12010-013-0301-4 (2013).
 <!-- Add more about the biology of this part here