Difference between revisions of "Part:BBa K3279008:Design"

Revision as of 15:27, 15 October 2019

cenA gene fused with INP-N sequence

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 333
Illegal BamHI site found at 733
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 75
Illegal NgoMIV site found at 408
Illegal AgeI site found at 1051
Illegal AgeI site found at 1414
1000
COMPATIBLE WITH RFC[1000]

Design Notes

The primary concern was BioBrick compatibility so that illegal sites in RFC[10] did not appear in the sequence. And the sequeces was finally designed to link with pET30a(+) , so we added restriction enzyme cutting site NdeI at the 5' and SalI at the 3'.

Source

cenA gene (BBa_K118023) is from Cellulomonas fimi genomic DNA. INP-N sequence (BBa_K3279006) is originally from Pseudomonas syringae genomic DNA and we (CAU_China 2019) did a codon optimization.

References

[1]Gu, M. Z., Wang, J. C., Liu, W. B., Zhou, Y. & Ye, B. C. Expression and displaying of beta-glucosidase from Streptomyces coelicolor A3 in Escherichia coli. Applied biochemistry and biotechnology 170, 1713-1723, doi:10.1007/s12010-013-0301-4 (2013). [2] Li Mingya, Lin Chenshui, Li Mingya, et al. Ice-nucleation Protein and Its Application in Bacterial surface Display Technology [J]. Amino Acids and Biological Resources, 2016, 38 (2): 7-11.

@@ Line 16: / Line 16: @@
 ===References===
+[1]Gu, M. Z., Wang, J. C., Liu, W. B., Zhou, Y. & Ye, B. C. Expression and displaying of beta-glucosidase from Streptomyces coelicolor A3 in Escherichia coli. Applied biochemistry and biotechnology 170, 1713-1723, doi:10.1007/s12010-013-0301-4 (2013).
+[2] Li Mingya, Lin Chenshui, Li Mingya, et al. Ice-nucleation Protein and Its Application in Bacterial surface Display Technology [J]. Amino Acids and Biological Resources, 2016, 38 (2): 7-11.