Difference between revisions of "Part:BBa K3096051"
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===Sequence and Features===
 
===Sequence and Features===
 
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===Usage and Biology===
 
  
  
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==References==
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===Functional Parameters===
 
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Revision as of 15:15, 15 October 2019

CRISPR array that targets the genomic DNA of E.coli - expression controlled via the Mnt repressor

The CRISPR array sequence needed to guide the Cas3 nuclease to its cut sites in the genome of E.coli under the control of the Mnt promoter. When Mnt is present the array is not transcribed and the CRISPR/Cas3 System lacks ist gRNA and is inactive. Combined with the GlcNAc-6-P responsive repression system (BBa_K3096048) the expression of the array can be controlled.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References