Difference between revisions of "Part:BBa K3112050"

Revision as of 15:12, 15 October 2019

mRFP reporter with weak constitutive promoter(J23115)

Compared with the original part: BBa_J04450, we replace the lac1 promoter that may leak with a member of a constituent promoter family (BBa_J23115). It has a weak expression intensity, but can still be clearly observed under fluorescence microscopy.

We hope that this part can be used in co-expression scenarios with some colored products, and its weak expression intensity will not affect the observation of its colored products.

In addition, two restriction sites EcoRI and BamHI were added to both ends of the whole part by PCR. It is for the convenience of other teams to carry out further cloning operations or add other constitutive promoters or terminators.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 4
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 4
Illegal NheI site found at 16
Illegal NheI site found at 39
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 4
Illegal BamHI site found at 924
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 4
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 4
Illegal AgeI site found at 635
Illegal AgeI site found at 747
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3112050 short</partinfo>
-Compared with the original part: <partinfo>BBa_J04450</partinfo>, We replace the lac1 promoter that may leak with a member of a constituent promoter family (<partinfo>BBa_J23115</partinfo>). It has a weak expression intensity, but can still be clearly observed under fluorescence microscopy.
+Compared with the original part: <partinfo>BBa_J04450</partinfo>, we replace the lac1 promoter that may leak with a member of a constituent promoter family (<partinfo>BBa_J23115</partinfo>). It has a weak expression intensity, but can still be clearly observed under fluorescence microscopy.
 We hope that this part can be used in co-expression scenarios with some colored products, and its weak expression intensity will not affect the observation of its colored products.