Difference between revisions of "Part:BBa K3241000:Design"

 
(→‎Source)
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
This gene is from Ralstonia eutropha.
+
This gene is originally from Ralstonia eutropha.GenScript synthesized the gene and optimized its codon for us.
−
 
+
  
 
===References===
 
===References===

Revision as of 15:02, 15 October 2019


PhbA(Beta-ketothiolase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1126
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 225
    Illegal NgoMIV site found at 310
    Illegal NgoMIV site found at 715
    Illegal NgoMIV site found at 844
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 141


Design Notes

atgaccgatgttgtcattgtctctgccgctcgtaccgccgttggtaaatttggtggcagcttggccaaaattccggctcctgaattgggcgccgttgtcattaaagccgctttggaacgtgctggtgttaaaccggaacaggtctctgaagttattatgggccaagtcttgaccgctggtagcggtcagaatcctgctcgtcaagccgctattaaagccggtttgccggctatggtccctgccatgaccattaataaagtttgtggctctggtttgaaagccgtcatgttggccgctaatgctattatggccggcgatgctgaaattgttgtcgctggtggccaggaaaatatgtctgccgctccgcatgttttgcctggcagccgtgatggttttcgcatgggcgatgccaaattggtcgataccatgattgttgatggtttgtgggatgtttataatcagtatcatatgggcatcaccgctgaaaatgtcgccaaagaatatggtattacccgcgaagcccaagatgaatttgctgttggtagccagaataaagccgaagccgctcaaaaagctggcaaatttgatgaagaaattgtcccggttttgattcctcagcgtaaaggtgatccggttgcctttaaaaccgatgaatttgtccgccaaggtgctaccttggattctatgagcggcttgaaacctgcttttgataaagccggcaccgttaccgccgctaatgcctctggtttgaatgatggcgccgctgccgttgtcgttatgagcgctgccaaagccaaagaattgggtttgaccccgttggctaccattaaatcttatgctaatgccggcgtcgatcctaaagttatgggcatgggtccggttcctgcttctaaacgtgccttgagccgcgctgaatggaccccgcaggatttggatttgatggaaattaatgaagcctttgctgcccaagctttggccgtccatcagcaaatgggttgggatacctctaaagtcaatgttaatggtggcgctattgccattggccatcctattggcgccagcggttgtcgtattttggttaccttgttgcatgaaatgaaacgtcgcgatgccaaaaaaggcttggctagcttgtgcattggtggcggtatgggtgtcgctttggccgttgaacgcaaataa


Source

This gene is originally from Ralstonia eutropha.GenScript synthesized the gene and optimized its codon for us.

References