Difference between revisions of "Part:BBa K3112051"

Revision as of 15:02, 15 October 2019

mRFP reporter with weak constitutive promoter(J23110)

Compared with the original part<a href=https://parts.igem.org/Part:BBa_J04450>(BBa_J04450)</a>，We replace the lac1 promoter that may leak with a member of a constituent promoter family(BBa_J23110). It has a weak expression intensity(weaker than BBa_K6112050), but can still be clearly observed under fluorescence microscopy. We hope that this part can be used in co-expression scenarios with some colored products(for example: lycopene and other β-carotene, which have a color of red or orange), and its weak expression intensity will not affect the observation of its colored products. In addition, two restriction sites EcoRI and BamHI were added to both ends of the whole part by PCR. It is for the convenience of other teams to carry out further cloning operations or add other constitutive promoters or terminators.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 5
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 5
Illegal NheI site found at 17
Illegal NheI site found at 40
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 5
Illegal BamHI site found at 925
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 5
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 5
Illegal AgeI site found at 636
Illegal AgeI site found at 748
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3112051 short</partinfo>
-Compared with the original part(BBa_J04450)&#65292;We replace the lac1 promoter that may leak with a member of a constituent promoter family(BBa_J23110). It has a weak expression intensity(weaker than BBa_K6112050), but can still be clearly observed under fluorescence microscopy.
+Compared with the original part<a href=https://parts.igem.org/Part:BBa_J04450>(BBa_J04450)</a>&#65292;We replace the lac1 promoter that may leak with a member of a constituent promoter family(BBa_J23110). It has a weak expression intensity(weaker than BBa_K6112050), but can still be clearly observed under fluorescence microscopy.
 We hope that this part can be used in co-expression scenarios with some colored products(for example: lycopene and other &#946;-carotene, which have a color of red or orange), and its weak expression intensity will not affect the observation of its colored products.
 In addition, two restriction sites EcoRI and BamHI were added to both ends of the whole part by PCR. It is for the convenience of other teams to carry out further cloning operations or add other constitutive promoters or terminators.