Difference between revisions of "Part:BBa K3096051"
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<partinfo>BBa_K3096051 short</partinfo> | <partinfo>BBa_K3096051 short</partinfo> | ||
| − | The CRISPR array sequence needed to guide the Cas3 nuclease to its cut sites in the genome of E.coli under the control of the Mnt promoter. When Mnt is present the array is not transcribed and the CRISPR/Cas3 System lacks ist gRNA and is inactive. Combined with the GlcNAc-6-P responsive repression system (BBa_K3096048) the expression of the array can be controlled. | + | The CRISPR array sequence needed to guide the Cas3 nuclease to its cut sites in the genome of E.coli under the control of the Mnt promoter. When Mnt is present the array is not transcribed and the CRISPR/Cas3 System lacks ist gRNA and is inactive. Combined with the GlcNAc-6-P responsive repression system (BBa_K3096048) the expression of the array can be controlled. |
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| + | ===Sequence and Features=== | ||
| + | <partinfo>BBa_K3096051 SequenceAndFeatures</partinfo> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
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Revision as of 14:50, 15 October 2019
CRISPR array that targets the genomic DNA of E.coli - expression controlled via the Mnt repressor
The CRISPR array sequence needed to guide the Cas3 nuclease to its cut sites in the genome of E.coli under the control of the Mnt promoter. When Mnt is present the array is not transcribed and the CRISPR/Cas3 System lacks ist gRNA and is inactive. Combined with the GlcNAc-6-P responsive repression system (BBa_K3096048) the expression of the array can be controlled.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]