Difference between revisions of "Part:BBa K3253001"

 
 
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<partinfo>BBa_K3253001 short</partinfo>
 
<partinfo>BBa_K3253001 short</partinfo>
  
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this is the primer we added to the gene in order to make it be successfully cut by the EcoR1 restriction enzyme
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This is the primer we added to the gene in order to make it be successfully cut by the EcoR1 restriction enzyme. The original sequence contains the wrong restriction site that cannot be fully cut and transferred to the plasmid. So we employ this primer with a specific restriction site to solve this problem.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 14:44, 15 October 2019


Gene-EcoR1-F

This is the primer we added to the gene in order to make it be successfully cut by the EcoR1 restriction enzyme. The original sequence contains the wrong restriction site that cannot be fully cut and transferred to the plasmid. So we employ this primer with a specific restriction site to solve this problem.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2
  • 1000
    COMPATIBLE WITH RFC[1000]