Difference between revisions of "Part:BBa K3096006"
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<partinfo>BBa_K3096006 short</partinfo> | <partinfo>BBa_K3096006 short</partinfo> | ||
Promoter that can be regulated by NagC which is a repressor for the nag-operon which is needed for GlcNAc6P catabolism. | Promoter that can be regulated by NagC which is a repressor for the nag-operon which is needed for GlcNAc6P catabolism. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Commensal bacteria in the gut metabolize the mucus within the intestines, which increases the level of GlcNAc within the lumen. GlcNAc is taken up by the bacteria through their PTS system and metabolized into GlcNAc-6-P, which binds the repressor protein nagC. If nagC is bound to GlcNAc-6-P it loses ist abilities to bind DNA, and therefore ist respective regulation activity. NagC can consequently not serve as a repressor of the pNagC controlled nagBACDE operon, anymore. | Commensal bacteria in the gut metabolize the mucus within the intestines, which increases the level of GlcNAc within the lumen. GlcNAc is taken up by the bacteria through their PTS system and metabolized into GlcNAc-6-P, which binds the repressor protein nagC. If nagC is bound to GlcNAc-6-P it loses ist abilities to bind DNA, and therefore ist respective regulation activity. NagC can consequently not serve as a repressor of the pNagC controlled nagBACDE operon, anymore. | ||
| + | ===Sequence and Features=== | ||
| + | <partinfo>BBa_K3096006 SequenceAndFeatures</partinfo> | ||
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==References== | ==References== | ||
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#'''Barnhart MM, Lynem J, Chapman MR.''' GlcNAc-6P levels modulate the expression of Curli fibers by Escherichia coli. ''J Bacteriol.'' ('''2006'''); 188(14):5212–5219. doi:10.1128/JB.00234-06 | #'''Barnhart MM, Lynem J, Chapman MR.''' GlcNAc-6P levels modulate the expression of Curli fibers by Escherichia coli. ''J Bacteriol.'' ('''2006'''); 188(14):5212–5219. doi:10.1128/JB.00234-06 | ||
#'''Konopka JB.''' N-acetylglucosamine (GlcNAc) functions in cell signaling. ''Scientifica (Cairo)''. ('''2012'''); 2012:489208. doi:10.6064/2012/489208 | #'''Konopka JB.''' N-acetylglucosamine (GlcNAc) functions in cell signaling. ''Scientifica (Cairo)''. ('''2012'''); 2012:489208. doi:10.6064/2012/489208 | ||
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<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
Latest revision as of 14:39, 15 October 2019
NagC regulated promoter
Promoter that can be regulated by NagC which is a repressor for the nag-operon which is needed for GlcNAc6P catabolism.
Usage and Biology
Commensal bacteria in the gut metabolize the mucus within the intestines, which increases the level of GlcNAc within the lumen. GlcNAc is taken up by the bacteria through their PTS system and metabolized into GlcNAc-6-P, which binds the repressor protein nagC. If nagC is bound to GlcNAc-6-P it loses ist abilities to bind DNA, and therefore ist respective regulation activity. NagC can consequently not serve as a repressor of the pNagC controlled nagBACDE operon, anymore.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
- Sicard JF, Le Bihan G, Vogeleer P, Jacques M, Harel J. Interactions of Intestinal Bacteria with Components of the Intestinal Mucus. Front Cell Infect Microbiol. (2017); 7:387. Published 2017 Sep 5. doi:10.3389/fcimb.2017.00387
- Barnhart MM, Lynem J, Chapman MR. GlcNAc-6P levels modulate the expression of Curli fibers by Escherichia coli. J Bacteriol. (2006); 188(14):5212–5219. doi:10.1128/JB.00234-06
- Konopka JB. N-acetylglucosamine (GlcNAc) functions in cell signaling. Scientifica (Cairo). (2012); 2012:489208. doi:10.6064/2012/489208