Difference between revisions of "Part:BBa K2965021"
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+ | __NOTOC__ | ||
+ | <partinfo>BBa_K2965021 short</partinfo> | ||
+ | AsCas12a (AsCpf1) is a programmable DNA endonuclease guided by a single CRISPR RNA (crRNA). Targeting requires a crRNA complementary to the target site as well as a 5' TTTN protospacer adjacent motif (PAM) on the DNA strand opposite the target sequence. Once it recognize its target, it will be activated and exhibits a “collateral effects” of promiscuous DNase activity. | ||
+ | |||
+ | <!-- Add more about the biology of this part here | ||
+ | ===Usage and Biology=== | ||
+ | |||
+ | <!-- --> | ||
+ | <span class='h3bb'>Sequence and Features</span> | ||
+ | <partinfo>BBa_K2965021 SequenceAndFeatures</partinfo> | ||
+ | |||
+ | |||
+ | <!-- Uncomment this to enable Functional Parameter display | ||
+ | ===Functional Parameters=== | ||
+ | <partinfo>BBa_K2965021 parameters</partinfo> | ||
+ | <!-- --> |
Revision as of 14:03, 15 October 2019
AsCas12a (AsCpf1)
AsCas12a (AsCpf1) is a programmable DNA endonuclease guided by a single CRISPR RNA (crRNA). Targeting requires a crRNA complementary to the target site as well as a 5' TTTN protospacer adjacent motif (PAM) on the DNA strand opposite the target sequence. Once it recognize its target, it will be activated and exhibits a “collateral effects” of promiscuous DNase activity.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 196
Illegal PstI site found at 1258
Illegal PstI site found at 3667
Illegal PstI site found at 3865 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 196
Illegal PstI site found at 1258
Illegal PstI site found at 3667
Illegal PstI site found at 3865 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 380
Illegal BglII site found at 1922
Illegal BglII site found at 2231 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 196
Illegal PstI site found at 1258
Illegal PstI site found at 3667
Illegal PstI site found at 3865 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 196
Illegal PstI site found at 1258
Illegal PstI site found at 3667
Illegal PstI site found at 3865
Illegal NgoMIV site found at 3466 - 1000COMPATIBLE WITH RFC[1000]