Difference between revisions of "Part:BBa K3221200"
| Line 5: | Line 5: | ||
GAL system is a galactose-mediated induction system in yeast. To be specific, GAL1 promoter can induce downstream gene expression under the control of GAL4 protein. What’s more, in order to simplify the verification of GAL1 promoter function, we chose the yeGFP as a reporter instead of Cns1 and Cns2 in our project. | GAL system is a galactose-mediated induction system in yeast. To be specific, GAL1 promoter can induce downstream gene expression under the control of GAL4 protein. What’s more, in order to simplify the verification of GAL1 promoter function, we chose the yeGFP as a reporter instead of Cns1 and Cns2 in our project. | ||
| − | [[File: T--SCU-China--scu-pGAldelay2019.png|450px|thumb|left|'''Fig.1 ''' A1 and B1: WT in YPG；A2 and B2 : Transformed in YPD；A3, A4, B3 and B4: Transformed in YPG. Visible Fluorescence in B3 and B4.]] | + | [[File: T--SCU-China--scu-pGAldelay2019.png|450px|thumb|left|'''Fig.1 Fluorescence observation''' A1 and B1: WT in YPG；A2 and B2 : Transformed in YPD；A3, A4, B3 and B4: Transformed in YPG. Visible Fluorescence in B3 and B4.]] |
Revision as of 12:27, 15 October 2019
Galactose-mediated induction system, yeGFP as a reporter
GAL system is a galactose-mediated induction system in yeast. To be specific, GAL1 promoter can induce downstream gene expression under the control of GAL4 protein. What’s more, in order to simplify the verification of GAL1 promoter function, we chose the yeGFP as a reporter instead of Cns1 and Cns2 in our project.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 150
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1199