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Revision as of 11:59, 15 October 2019
mCherry Fluorescence Protein
| name | coat protein with LPETGG in pET24 |
| base pairs | 705 |
| molecular weight | 26.6 kDa |
| origin | synthetic, derived from Discosoma sp. |
| properties | Red fluorescent, Ex λ: 587nm, Em λ: 610 nm |
mCherry is a red fluorescent protein, which is derived from Discosoma sp.. It absorbs light with a wavelength of 587 nm and emits light with a wavelength of 610 nm. mCherry can be modified N- and C-terminal without a deficit in fluorescent.[1] https://2019.igem.org/wiki/images/c/c6/T--TU_Darmstadt--Registry_mCherry.jpg
Figure 1: Crystal structure of mCherry.
References
- ↑ Nathan Shaner, Robert Campbell, Paul Steinbach, Ben Giepmans, Amy Palmer and Roger Tsien, Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein, Nature Biotechnology, 2004, 22: 1567-1572 [1]
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
- <a href="#cite_ref-1">↑</a> Nathan Shaner, Robert Campbell, Paul Steinbach, Ben Giepmans, Amy Palmer and Roger Tsien, Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein, Nature Biotechnology, 2004, 22: 1567-1572 <a rel="nofollow" class="external autonumber" href="#https://www.nature.com/articles/nbt1037">[1] </a>
Sequence and Features
Assembly Compatibility: