Difference between revisions of "Part:BBa K3187026"
Line 27: Line 27:
 
<br>
 
<br>
  
−
mCherry is a red fluorescent protein, which is derived from <i>Discosoma</i> sp.. It absorbs light with a wavelength of 587 nm and emits light with a wavelength of 610 nm. mCherry can be modified N- and C-terminal without a deficit in fluorescent.<sup id="cite_ref-1" class="reference">
+
mCherry is a red fluorescent protein, which is derived from <i>Discosoma</i> sp.. It absorbs light with a wavelength of 587 nm and emits light with a wavelength of 610 nm. mCherry can be modified N- and C-terminal without a deficit in fluorescent.<sup id="cite_ref-1" class="reference"><a href="#cite_note-1">[1] </a> </sup>
−
                    <a href="#cite_note-1">[1] </a> </sup>
+
  
 
https://2019.igem.org/wiki/images/c/c6/T--TU_Darmstadt--Registry_mCherry.jpg
 
https://2019.igem.org/wiki/images/c/c6/T--TU_Darmstadt--Registry_mCherry.jpg

Revision as of 11:54, 15 October 2019


mCherry Fluorescence Protein

name coat protein with LPETGG in pET24
base pairs 705
molecular weight 26.6 kDa
origin synthetic, derived from Discosoma sp.
properties Red fluorescent, Ex λ: 587nm, Em λ: 610 nm


mCherry is a red fluorescent protein, which is derived from Discosoma sp.. It absorbs light with a wavelength of 587 nm and emits light with a wavelength of 610 nm. mCherry can be modified N- and C-terminal without a deficit in fluorescent.<a href="#cite_note-1">[1] </a>

T--TU_Darmstadt--Registry_mCherry.jpg

Figure 1: Crystal structure of mCherry.


References

  1. <a href="#cite_ref-1">↑</a> Nathan Shaner, Robert Campbell, Paul Steinbach, Ben Giepmans, Amy Palmer and Roger Tsien, Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein, Nature Biotechnology, 2004, 22: 1567-1572 <a rel="nofollow" class="external autonumber" href="#https://www.nature.com/articles/nbt1037">[1] </a>

    2. Sequence and Features


    Assembly Compatibility:
    • 10
      COMPATIBLE WITH RFC[10]
    • 12
      COMPATIBLE WITH RFC[12]
    • 21
      COMPATIBLE WITH RFC[21]
    • 23
      COMPATIBLE WITH RFC[23]
    • 25
      COMPATIBLE WITH RFC[25]
    • 1000
      COMPATIBLE WITH RFC[1000]


    References

    1. <a href="#cite_ref-1">↑</a> Nathan Shaner, Robert Campbell, Paul Steinbach, Ben Giepmans, Amy Palmer and Roger Tsien, Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein, Nature Biotechnology, 2004, 22: 1567-1572 <a rel="nofollow" class="external autonumber" href="#https://www.nature.com/articles/nbt1037">[1] </a>