Difference between revisions of "Part:BBa K2999003"

Revision as of 11:34, 15 October 2019

ptac+RBS+PelA+RBS+PslG

Biofilm is a complex bacterial community embedded in the extracellular matrix composed of proteins, extracellular DNA (eDNA) and extracellular polysaccharides. Pseudomonas aeruginosa PAO1 has two types of biofilm, Psl and Pel. This part is mainly used for encoding polysaccharide hydrolases PslG and PelA, and destroying the structure of biofilm.

YAU-China expressed the biofilm-damaged system in 2019, using extracts from each part of the system for experiments. After overnight induction of the control strain, two strains expressing single enzyme and two strains co-expressing two enzymes, four strains were collected by centrifugation and suspended in PBS buffer of equal volume. The supernatant was absorbed and added into the biofilm of Pseudomonas aeruginosa cultured on the pre-PVC plate. After incubation for a period of time, the PVC plate was cleaned, stained with crystal violet for 10 minutes, and the remaining biomass was treated with anhydrous ethanol. The coatings were washed out and OD570 was detected by enzyme labeling.

Figure 1. Effects of PelA and PslG on Biofilm

Figure 2. Crystal violet staining of biofilm treated with extracts from control group and three groups