Difference between revisions of "Part:BBa K3111103:Design"
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===Source=== | ===Source=== | ||
| − | Encapsulin was isolated from Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099). Sequence was taken from <partinfo>BBa_K2686002</partinfo> (without the stop codon as that would disable fusion on the C-terminus). | + | Encapsulin was isolated from <I>Thermotoga maritima</i> (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099). Sequence was taken from <partinfo>BBa_K2686002</partinfo> (without the stop codon as that would disable fusion on the C-terminus). |
===References=== | ===References=== | ||
Moon, H., Lee, J., Min, J. and Kang, S. (2014). Developing Genetically Engineered Encapsulin Protein Cage Nanoparticles as a Targeted Delivery Nanoplatform. Biomacromolecules, 15(10), pp.3794-3801. | Moon, H., Lee, J., Min, J. and Kang, S. (2014). Developing Genetically Engineered Encapsulin Protein Cage Nanoparticles as a Targeted Delivery Nanoplatform. Biomacromolecules, 15(10), pp.3794-3801. | ||
Latest revision as of 11:27, 15 October 2019
T. maritima encapsulin (6-His) with a StrepII-tag expressed under T7 promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 183
Illegal BglII site found at 598 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1013
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 532
Illegal SapI.rc site found at 563
Design Notes
-
Source
Encapsulin was isolated from Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099). Sequence was taken from BBa_K2686002 (without the stop codon as that would disable fusion on the C-terminus).
References
Moon, H., Lee, J., Min, J. and Kang, S. (2014). Developing Genetically Engineered Encapsulin Protein Cage Nanoparticles as a Targeted Delivery Nanoplatform. Biomacromolecules, 15(10), pp.3794-3801.