Difference between revisions of "Part:BBa K2922038"

Revision as of 11:01, 15 October 2019

The colicin-N operon under T7 promoter control with a gfasPurple chromoprotein reporter

Summary

This is a composite part consist of a T7 promoter (BBa_K525998), CDS of colicin-N (BBa_K2922027), CDS of colicin-N immunity protein (BBa_K2922028), CDS of lysis protein (BBa_K2922029) and a gfasPurple chromoprotein reporter(BBa_K1033917) . Each CDS has an RBS (BBa_B0034) behind. T7 promoter could be induced by IPTG or lactose in Escherichia coli BL21 strain and then express all proteins mentioned. E coli that can`t express colicin-N immunity protein would be killed by colicin-N, colicin-N immunity protein is used to protect itself from attack of extracellular colicin-N and lysis protein helps the release of colicin-N. The gfasPurple chromoprotein reporter is used to present the growth curve specifically of strain which contains this part. This part is constructed in the aim of achieve our “aggressive” design.

Ientification

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1938
Illegal NheI site found at 1961
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 598
Illegal AgeI site found at 1602
1000
COMPATIBLE WITH RFC[1000]

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 ===Summary===
-This is a composite part consist of a T7 promoter (<partinfo>BBa_K525998</partinfo>), CDS of colicin-N (<partinfo>BBa_K2922027</partinfo>), CDS of colicin-N immunity protein (<partinfo>BBa_K2922028</partinfo>), CDS of lysis protein (<partinfo>BBa_K2922029</partinfo>) and the gfasPurple chromoprotein reporter(<partinfo>BBa_K1033917</partinfo>) . Each CDS has an RBS (<partinfo>BBa_B0034</partinfo>) behind. T7 promoter could be induced by IPTG or lactose in Escherichia coli BL21 strain and then express all proteins mentioned. E coli that can`t express colicin-N immunity protein would be killed by colicin-N, colicin-N immunity protein is used to protect itself from attack of extracellular colicin-N and lysis protein helps the release of colicin-N. The gfasPurple chromoprotein reporter is used to present the growth curve specifically of strain which contains this part. This part is constructed in the aim of achieve our “aggressive” design.
+This is a composite part consist of a T7 promoter (<partinfo>BBa_K525998</partinfo>), CDS of colicin-N (<partinfo>BBa_K2922027</partinfo>), CDS of colicin-N immunity protein (<partinfo>BBa_K2922028</partinfo>), CDS of lysis protein (<partinfo>BBa_K2922029</partinfo>) and a gfasPurple chromoprotein reporter(<partinfo>BBa_K1033917</partinfo>) . Each CDS has an RBS (<partinfo>BBa_B0034</partinfo>) behind. T7 promoter could be induced by IPTG or lactose in Escherichia coli BL21 strain and then express all proteins mentioned. E coli that can`t express colicin-N immunity protein would be killed by colicin-N, colicin-N immunity protein is used to protect itself from attack of extracellular colicin-N and lysis protein helps the release of colicin-N. The gfasPurple chromoprotein reporter is used to present the growth curve specifically of strain which contains this part. This part is constructed in the aim of achieve our “aggressive” design.
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