Difference between revisions of "Part:BBa K3187016"
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| − | + | <p>Sortase A5M (SrtA5M) is a catalytically optimized transpeptidase derived from the enzyme Sortase A from Staphylococcus aureus.1 Similar to Sortase A, SrtA5M connects two peptides covalently, one with a poly-glycine (poly-G) at the N-terminus and one with LPXTG motif at the C-terminus. It acts by cleaving between Gly and Thr of the LPXTG motif and catalysing the formation of an amide bond to the poly-G sequence.2 For the expression and purification of SrtA5M, a part containing T7 promoter, a lac operator and a ribosomal binding site upstream and a Strep-Tag II downstream of the coding sequence were added.</p> | |
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 10:21, 15 October 2019
Sortase A5M
Sortase A5M (SrtA5M) is a catalytically optimized transpeptidase derived from the enzyme Sortase A from Staphylococcus aureus.1 Similar to Sortase A, SrtA5M connects two peptides covalently, one with a poly-glycine (poly-G) at the N-terminus and one with LPXTG motif at the C-terminus. It acts by cleaving between Gly and Thr of the LPXTG motif and catalysing the formation of an amide bond to the poly-G sequence.2 For the expression and purification of SrtA5M, a part containing T7 promoter, a lac operator and a ribosomal binding site upstream and a Strep-Tag II downstream of the coding sequence were added.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]