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(Experimental Results)
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   <p>Figure 1 The ration of ONPG is 1:2,1:4,1:8,1:10,1:15,1:20 from the left to right.</p >
 
   <p>Figure 1 The ration of ONPG is 1:2,1:4,1:8,1:10,1:15,1:20 from the left to right.</p >
 
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Revision as of 09:13, 15 October 2019


LacZ alpha

An alpha peptide chain encoded by a gene LacZ


Usage and Biology

The LacZ alpha gene is one of the structural genes present on the Lac Operon in Escherichia coli bacteria . When transcribed by RNA polymerase , the LacZ alpha gene codes for β-galactosidase , an enzyme responsible for the hydrolysis of β-galactosides (e.g. 2-Nitrophenyl β-D-galactopyranoside (ONPG) )

Characterize

LacZ alpha gene encodes the β-galactosidase, which could react with 2-Nitrophenyl β-D-galactopyranoside (ONPG) to generate orange color.
Mechanism: T--ZJUT-China--igem-part07jili.png

Experimental Results

Form 1 The absorbance of different reaction solution at the wavelength of350 nm

< img src="https://2019.igem.org/wiki/images/5/5a/T--ZJUT-China--igem-part07form.png" alt=""width="600px">

Figure 1 The ration of ONPG is 1:2,1:4,1:8,1:10,1:15,1:20 from the left to right.