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===<h1>Experimental Results</h1>===
 
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[[File:T--ZJUT-China--igem-part07form.png|600px|thumb|left|Form 1 The absorbance of different reaction solution at the wavelength of350 nm]]
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[[File:T--ZJUT-China--igem-part07reaction.jpg|600px|thumb|left|Figure1 The ration of ONPG is 1:2,1:4,1:8,1:10,1:15,1:20 from the left to right.]]
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  <p>Figure 1 The ration of ONPG is 1:2,1:4,1:8,1:10,1:15,1:20 from the left to right.</p >
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 09:03, 15 October 2019


LacZ alpha

An alpha peptide chain encoded by a gene LacZ


Usage and Biology

The LacZ alpha gene is one of the structural genes present on the Lac Operon in Escherichia coli bacteria . When transcribed by RNA polymerase , the LacZ alpha gene codes for β-galactosidase , an enzyme responsible for the hydrolysis of β-galactosides (e.g. 2-Nitrophenyl β-D-galactopyranoside (ONPG) )

Characterize

LacZ alpha gene encodes the β-galactosidase, which could react with 2-Nitrophenyl β-D-galactopyranoside (ONPG) to generate orange color.
Mechanism: T--ZJUT-China--igem-part07jili.png

Experimental Results

Form 1 The absorbance of different reaction solution at the wavelength of350 nm

< img src="https://2019.igem.org/wiki/images/5/5a/T--ZJUT-China--igem-part07form.png" alt=""width="600px"> < img src="https://2019.igem.org/wiki/images/thumb/5/57/T--ZJUT-China--igem-part07reaction.jpg/800px-T--ZJUT-China--igem-part07reaction.jpg" alt=""width="600px">

Figure 1 The ration of ONPG is 1:2,1:4,1:8,1:10,1:15,1:20 from the left to right.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]