Difference between revisions of "Part:BBa K3239005"

Revision as of 08:50, 15 October 2019

Prm1 transcription factor in the P.pastoris GS115 strain

Transcription factor in the P. pastoris GS115 strain. Self-upregulates Prm1 expression and upregulates Mit1 and AOX1 expression.

Usage and Biology

The AOX1 promoter is homogenous to the P. pastoris GS115 strain and is critical in the regulation of AOX1 (alcohol oxidase) expression in the methylotrophic yeast. Upon methanol induction, the Mxr1 protein is expressed and the PRM1 promoter is activated. The Mxr1 protein derepresses AOX1 promoter activation, whereas the Prm1 protein not only self-upregulates but also upregulates the expression of the Mit1 protein. Prm1 and Mit1 both upregulate AOX1 expression, allowing the yeast to instantly produce enough alcohol oxidase to metabolize methanol. Mit1 however, also downregulates the expression of Prm1, inhibiting the over-activation of Prm1 expression and subsequent Mit1 and alcohol oxidase expression.

Reference

Liang, S., Wang, B., Pan, L., Ye, Y., He, M., Han, S., … Lin, Y. (2012). Comprehensive structural annotation of Pichia pastoris transcriptome and the response to various carbon sources using deep paired-end RNA sequencing. BMC Genomics, 13(1). https://doi.org/10.1186/1471-2164-13-738

Shi, L., Wang, J., Wang, X., Zhang, Y., Song, Z., Cai, M., & Zhou, X. (2019). Transcriptome and metabolome analyses reveal global behaviour of a genetically engineered methanol-independent Pichia pastoris strain. Process Biochemistry, 76(August 2018), 46–54. https://doi.org/10.1016/j.procbio.2018.10.014

Wang, X., Wang, Q., Wang, J., Zhou, M., Shi, L., Zhou, X., … Shen, W. (2016). Mit1 Transcription Factor Mediates Methanol Signaling and Regulates the Alcohol Oxidase 1 ( AOX1 ) Promoter in Pichia pastoris. Journal of Biological Chemistry, 291(12), 6245–6261. https://doi.org/10.1074/jbc.m115.692053

Sequence and Features