Difference between revisions of "Part:BBa K3279007"
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β-Glucosidase obtained from various sources has been used in many areas, such as the enzymatic saccharification of cellulosic materials, the liberation of flavor compounds in fruit juices and wines, and so on. In cellulose hydrolysis, β-glucosidase is used to degrade cello-oligosaccharides to glucose[1]. We used this part to accomplish the cellulose degradation.
 
β-Glucosidase obtained from various sources has been used in many areas, such as the enzymatic saccharification of cellulosic materials, the liberation of flavor compounds in fruit juices and wines, and so on. In cellulose hydrolysis, β-glucosidase is used to degrade cello-oligosaccharides to glucose[1]. We used this part to accomplish the cellulose degradation.
  
 +
====References====
 
[1]Gu, M. Z., Wang, J. C., Liu, W. B., Zhou, Y. & Ye, B. C. Expression and displaying of beta-glucosidase from Streptomyces coelicolor A3 in Escherichia coli. Applied biochemistry and biotechnology 170, 1713-1723, doi:10.1007/s12010-013-0301-4 (2013).
 
[1]Gu, M. Z., Wang, J. C., Liu, W. B., Zhou, Y. & Ye, B. C. Expression and displaying of beta-glucosidase from Streptomyces coelicolor A3 in Escherichia coli. Applied biochemistry and biotechnology 170, 1713-1723, doi:10.1007/s12010-013-0301-4 (2013).
  

Revision as of 06:27, 15 October 2019


β-Glucosidase from Streptomyces coelicolor

β-Glucosidase obtained from various sources has been used in many areas, such as the enzymatic saccharification of cellulosic materials, the liberation of flavor compounds in fruit juices and wines, and so on. In cellulose hydrolysis, β-glucosidase is used to degrade cello-oligosaccharides to glucose[1]. We used this part to accomplish the cellulose degradation.

References

[1]Gu, M. Z., Wang, J. C., Liu, W. B., Zhou, Y. & Ye, B. C. Expression and displaying of beta-glucosidase from Streptomyces coelicolor A3 in Escherichia coli. Applied biochemistry and biotechnology 170, 1713-1723, doi:10.1007/s12010-013-0301-4 (2013).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 287
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 427
    Illegal NgoMIV site found at 1103
    Illegal AgeI site found at 184
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 687
    Illegal BsaI.rc site found at 946