Difference between revisions of "Part:BBa K3110042:Design"

 
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
sfGFP was obtained from the Biobrick. lldRO1-J23100-lldRO2 were obtained as gBlocks from IDT and these were joined together using SOEing (Spliced Overlap Extenstion) PCR.
+
sfGFP was obtained from the Biobrick. lldRO1-J23117-lldRO2+B0033 was obtained as gBlock from IDT and these were joined together using SOEing (Spliced Overlap Extenstion) PCR.
  
  
  
 
===References===
 
===References===

Revision as of 05:04, 15 October 2019


lldRO1-J23117-lldRO2 Weak RBS sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 78
    Illegal NheI site found at 101
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 173


Design Notes

None.


Source

sfGFP was obtained from the Biobrick. lldRO1-J23117-lldRO2+B0033 was obtained as gBlock from IDT and these were joined together using SOEing (Spliced Overlap Extenstion) PCR.


References