Difference between revisions of "Part:BBa K3096040"
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| − | + | A constitutively active promoter expresses Clts, a temperature sensitive cI repressor of the lambda phage. If the temperature is significantly below 37°C (body temperature), Clts is an active repressor for the cI lambda promoter. This promoter controls the transcription of a repressor protein, AraC. Therefore, if conditions are not 37°C, the AraC is not available, hence the pBAD promoter, controlled by AraC, is active and genes controlled by the pBAD promoter are transcribed. | |
Revision as of 00:33, 15 October 2019
A constitutively active promoter expresses Clts, a temperature sensitive cI repressor of the lambda phage. If the temperature is significantly below 37°C (body temperature), Clts is an active repressor for the cI lambda promoter. This promoter controls the transcription of a repressor protein, AraC. Therefore, if conditions are not 37°C, the AraC is not available, hence the pBAD promoter, controlled by AraC, is active and genes controlled by the pBAD promoter are transcribed.