Difference between revisions of "Part:BBa K3021004:Design"

 
 
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===Design Notes===
 
===Design Notes===
−
In these, we have further optimized the Laccase and NSP4 sequence.
 
−
 
  
 +
Laccase sequence is codon optimized from previous iGEM team, which was initiated based on Trametes versicolor; and the secretion peptide, NSP4, is also codon optimized, sequence from E.coli based from a previous publication.
  
 
===Source===
 
===Source===
−
 
 
The original NSP4 sequence from the paper is ATGAAAAAGATTACCGCTGCTGCTGGTCTGCTGCTCCTCGCTGCCCAGCCGGCGATGGCG with the Protein sequence is The n-region: MKKI(the positive net charge in the n-region: +2) The h-region: TAAAGLLLLA The c-region: AQPAMA; We have codon optimized this sequence and also the Laccase1326 sequence, which is from previous publication.  
 
The original NSP4 sequence from the paper is ATGAAAAAGATTACCGCTGCTGCTGGTCTGCTGCTCCTCGCTGCCCAGCCGGCGATGGCG with the Protein sequence is The n-region: MKKI(the positive net charge in the n-region: +2) The h-region: TAAAGLLLLA The c-region: AQPAMA; We have codon optimized this sequence and also the Laccase1326 sequence, which is from previous publication.  
  
 
===References===
 
===References===
 +
References: Han, S., Machhi, S., Berge, M., Xi, G., Linke, T., & Schoner, R. (2017). Novel signal peptides improve the secretion of recombinant Staphylococcus aureus Alpha toxinH35L in Escherichia coli. AMB Express, 7, 93.

Latest revision as of 00:30, 15 October 2019


NSP4-Laccase1326-HisTag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1493
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1007
    Illegal BamHI site found at 1526
    Illegal XhoI site found at 227
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 136
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 862


Design Notes

Laccase sequence is codon optimized from previous iGEM team, which was initiated based on Trametes versicolor; and the secretion peptide, NSP4, is also codon optimized, sequence from E.coli based from a previous publication.

Source

The original NSP4 sequence from the paper is ATGAAAAAGATTACCGCTGCTGCTGGTCTGCTGCTCCTCGCTGCCCAGCCGGCGATGGCG with the Protein sequence is The n-region: MKKI(the positive net charge in the n-region: +2) The h-region: TAAAGLLLLA The c-region: AQPAMA; We have codon optimized this sequence and also the Laccase1326 sequence, which is from previous publication.

References

References: Han, S., Machhi, S., Berge, M., Xi, G., Linke, T., & Schoner, R. (2017). Novel signal peptides improve the secretion of recombinant Staphylococcus aureus Alpha toxinH35L in Escherichia coli. AMB Express, 7, 93.